Table 1. Antibodies used for immunofluorescence detection.
Antigen | Raised in | Dilution | Source | Marker of |
---|---|---|---|---|
NeuN | Mouse | 1:125 | Millipore | Neuron |
GFAP | Rabbit | 1:500 | Dako Cytomation | Astrocyte |
GFP | Chicken | 1:250 | Abcam Inc. | GFP |
Sox2 | Rabbit | 1:250 | Millipore | Neuronal precursor |
Olig2 | Mouse | 1:1000 | Millipore | Oligodendrocyte |
DCX | Mouse | 1:250 | Abcam Inc. | Neuroblast |
GAD67 | Mouse | 1:1000 | Millipore | Inhibitory neuron |
Calretinin | Rabbit | 1:1000 | Millipore | Inhibitory neuron |
Somatostatin | Rat | 1:200 | Millipore | Inhibitory neuron |
BLBP | Rabbit | 1:1000 | Abcam Inc. | Stem cells |