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Effect of time of incubation on reduction and alkylation of proteins in immobilized pH gradient strips after isoelectric focusing: (A) 20 minutes, (B) 40 minutes, and (C) 60 minutes. Dithiothreitol (2%) and iodoacetamide (2.5%) were used as reducing and alkylating agents, respectively. The details are given in the “Materials and Methods” section.
