Levels of miR-4417 and TRIM35 in HCC cell lines. (A) Levels of miR-4417 were measured by qRT-PCR and normalized to U6 snRNA expression in 3 HCC cell lines and 1 normal liver cell line. miR-4417 expression was upregulated in HCC cell lines Huh7, SMMC-7721, and SK-Hep1 as compared with normal liver cell line HL-7702. (B, C) TRIM35 expression was determined by qRT-PCR and Western blot, and normalized to levels of GAPDH and β-actin, respectively. The expression levels of TRIM35 mRNA and protein were downregulated in HCC cell lines compared to normal liver cell line HL-7702. ** P<0.01; *** P<0.001.