Figure 2.

Effects of miR-4417 and TRIM35 on proliferation and apoptosis of SMMC-7721 cells. (A, B) Cell growth and proliferation were measured by MTT and BrdU assays, respectively. Cell growth and proliferation were suppressed by miR-4417 inhibitor. (C) Cell apoptosis was measured by flow cytometry. miR-4417 inhibitor significantly promoted cell apoptosis compared with inhibitor control. (D–F) Upregulation of TRIM35 resulted in significant inhibitions in cell growth and proliferation, and a marked increase in apoptosis in SMMC-7721 cells. (G–F) miR-4417 inhibitor suppressed cell growth and proliferation, and promoted apoptosis in SMMC-7721 cells; however, si-TRIM35 significantly reversed these effects. * P<0.05; ** P<0.01; *** P<0.001.