Figure 3.

TRIM35 was a direct target of miR-4417 in SMMC-7721 cells. (A) Predicted miR-4417 target sequence in the 3′UTR of TRIM35 by online software, including TargetScan and miRanda. (B) Luciferase reporter assay showed the relative luciferase activities of TRIM35-WT and TRIM35-MUT co-transfected with miR-4417 mimics (miR-4417) or corresponding control (miR-control). (C, D) After SMMC-7721 cells were transfected with miR-4417 inhibitor, TRIM35 mRNA and protein levels were measured by qRT-PCR and Western blot, respectively. miR-4417 downregulation in SMMC-7721 cells was associated with increased TRIM35 mRNA and protein levels. ** P<0.01; *** P<0.001.