Figure 4.

miR-4417 and TRIM35 regulated SMMC-7721 cell proliferation and apoptosis by regulating PKM2 phosphorylation. (A, B) miR-4417 inhibitor and TRIM35 overexpression significantly blocked PKM2 Y105 phosphorylation but not the total PKM2 levels compared with their corresponding controls. (C) The inhibitory effect of miR-4417 inhibitor on PKM2 Y105 phosphorylation was overturned by si-TRIM35. (D–F) SMMC-7721 cells transfected with PKM2 Y105F showed lower viable cell numbers and proliferation rate, and a higher apoptosis rate compared with that with PKM2 Y105 phosphorylation. * P<0.05; ** P<0.01; *** P<0.001.