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CARD-FISH-MAR for thaumarchaeotes in RBC 1 (a and b) and RBC 8 (c and d) biofilm. FISH-MAR for AOB (orange/yellow) and Nitrospira (blue) (e and f; both RBC 8). Green signals in e and f originate from the EUB338 probe mix targeting most bacteria. Microautoradiographic images were taken with the CCD black/white camera integrated in the CLSM (e and f) or with an external CCD color camera (DFC 450, Leica Microsystem, Wetzlar, Germany; b and d). All scale bars represent 10 μm.
