Fig. 5.

In vivo intestinal effects of suberoylanilide hydroxamic acid (SAHA) in mice treated for five consecutive days and sacrificed on day 5, 3 hours after the oral administration, in terms of histone H3 acetylation (Ac-H3), poly-(ADP-ribose) polymerase (PARP), and heat shock protein 70 (Hsp70) protein expression, verified by immunoblot analysis with α-tubulin expression as a loading control (shown for two mice in each treatment group) (A), body weight change relative to the start of experiment on day 1 (group average values with standard error of the mean; ^*p < 0.05, ^**p < 0.01) (B) and histology of tissue rolls of intestinal sections stained with hematoxylin and eosin (H&E) or terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) (all images shown are at ×10 magnification and representative of each treatment group) (C).