Fig. 4.

Assessments of cell viability (A) and apoptosis (B) following prior blocking of IL4Rα receptors on 4T1 cells. Blocking was performed for 1 hour with either anti-IL4Rα antibody or isotype IgG antibody (i.e., without blocking) and assessments were performed after 12 hours of incubation following treatment with SPION-IgG1, anti-IL4Rα antibodies alone, SPION-IL4Rα, DOX, and SPION-IL4Rα+DOX. Data are expressed as the mean±standard error, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001. NT, no treatment; SPION-IL4Rα, superparamagnetic iron oxide nanoparticles conjugated with anti–interleukin-4 receptor α [IL4Rα] blocking antibodies; DOX, doxorubicin.