Fig. 3.

Content of c-fos and phosphorylated c-fos in the telencephalon of rats gestated in hypothyroxinemic mothers. A, Representative pictures of Western blots for c-fos and phosphorylated c-fos (phospho c-fos) from total telencephalon homogenates. These samples corresponded to total telencephalon homogenate of the MMIo or control (Co) group subjected (+) or not (−) to a water maze test on the second day. To analyze that the same amount of protein was loaded in each well, a Western blot analysis of actin was performed with the same nitrocellulose used for c-fos and phosphorylated c-fos antibodies. B, Ratio of c-fos or phosphorylated c-fos (phosphor c-fos) of rats subjected to the water maze test over those that were not. The intensities of c-fos or phosphorylated c-fos bands from the Western blots were measured by densitometry. The ratios between the intensity of c-fos or phosphorylated c-fos bands of rats subjected to the water maze test over those that were not were plotted. The statistical analysis indicated that the Co increased the content of phosphorylated c-fos after the second day of the water maze test (n = 3; *, P < 0.05). MW, Molecular weight.