Fig 1. MnP treatment effectively scavenges NADPH oxidase and mitochondrial derived superoxide, while demonstrating no toxicity.
NOD splenocytes were pre-treated with or without MnP and then loaded with either Dihydroethidium (DHE; a) or MitoSOX red (b). Splenocytes were stimulated with PMA and ionomycin and read for fluorescence by flow cytometry at the indicated time points. Data are displayed as delta mean fluorescence intensity (Δ MFI) ± SEM calculated as MFIstimulated − MFIunstimulated. (c and d) BDC2.5.TCR.Tg splenocyte cultures were stained for 7AAD and CD4 to assess viability of cultures due to MnP treatment. Data are displayed as percent 7AAD- of whole splenocytes (c) and CD4+ T cells (d). Significance was determined by Two-way ANOVA with Bonferroni post-hoc analysis of a combined n = 3–5 mice (**** = p<0.0001; *** = p<0.001; ** = p<0.01; *p<0.05).