Fig 6. AT injection increased phenoloxidase (PO) activity in Ae. aegypti hemolymph.

Mosquitoes were inoculated with 0.25 μl of PBS alone (C) or with 10⁻⁷ M of Aedes-AT (AT) or NPLP1 (NPL). After 1 h, hemolymph was obtained by perfusion and PO activity was evaluated. PO activity units represent the amount of enzyme required to produce an increase in absorbance of 0.001/min/mg of protein. Bars represent the means ± SEM of three independent replicates of groups of 10 mosquitoes. Significant differences were determined with one-way ANOVA followed by Tukey’s test (p < 0.05). Different letter on the top each bar, are significantly different.