Fig 8. AT induced antibacterial gene expression in An. albimanus cultured guts.

An. albimanus guts were incubated for 24h in medium alone (C) or with 10⁻⁷ M of Aedes-AT (AT) or E. coli lipopolysaccharide (LPS) (100 μg/ml). Relative expression of gambicin, attacin, and cecropin genes were measured by qPCR. Two independent experiments using three replicates per sample were performed and RNA (relative expression) represents the average fold-change relative to the control group (X_T/X_C). Significant differences were determined with one-way ANOVA followed by Tukey’s test (p < 0.05).