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. Author manuscript; available in PMC: 2018 Apr 15.
Published in final edited form as: J Immunol. 2017 Mar 8;198(8):3237–3244. doi: 10.4049/jimmunol.1601420

Figure 3. C5aA and C3aA suppress Ifnb message in BMDCs.

Figure 3

WT BMDCs were pre-treated with either media, 100 nM C5aA, or 100 nM C3aA for 2 h and then the cells were (A) infected with Lm or (B) incubated with 25 μg/ml c-di-AMP for 4 h. RNA was harvested from whole cell lysates, and message for Ifnb and Gapdh was quantitated from cDNA. Message for Ifnb was normalized to Gapdh for each sample, and each treatment group was compared to cells treated with only media (set as 1). Data is presented as mean ± SEM. These data are pooled from three independent experiments. * P ≤ 0.048; ** P = 0.003 by t test.