Fig 2. Secretion of the major collagens, Col I, III, and V, detected in the media following exposure to normoxic or hypoxic (2% O₂) conditions.

(A) Representative western blots and (B-D) quantification by densitometry showing a reduction in Col I in both HCFs and HKCs following hypoxia exposure. Error bars represent standard error of the mean. n = 3 for Col I and III, n = 2 for Col V. A two-way ANOVA was used to determine statistical significance with *p≤0.05, **p≤0.01, ***p≤0.001, and ****p≤0.0001.