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. 2013 Sep 24;154(12):4803–4813. doi: 10.1210/en.2013-1168

Figure 6.

Figure 6.

Effects of activation of PPARγ on expression of COX-2 mRNA and PGE2 biosynthesis in human endometriotic epithelial cells 12Z and stromal cells 22B. (A) Expression of COX-2 mRNA in 12Z and 22B cells. (B) PGE2 production in 12Z and 22B cells measured by ELISA in the culture media, n = 3. (C) Effects of activation of PPARγ on expression of EP2 receptor mRNA and in 12Z and 22B cells. (D) Effects of activation of PPARγ on expression of EP4 receptor mRNA and in 12Z and 22B cells. Expression of COX-2, EP2, or EP4 mRNA was performed using qPCR, and data were analyzed using dCT method. Numerical data are expressed in ratio between COX-2, EP2, or EP4 mRNA and β-actin mRNA as mean ± SEM of 3 (n = 3) independent experiments. CONT, control. The 12Z and 22B cells were treated with PPARγ agonist CTZ 25 μm for 24 hours. More details are provided in Materials and Methods and Result sections.