Table 2. Proportion of unmethylated/methylated fragments of L. terrestris coelomocytes.
Replica I | Time point (weeks) | Unmethylated | External C methylation | Internal C methylation | Hypermethylated |
Control | 0 | 31.98±1.68 | 4.19±0.96 | 6.63±1.24 | 57.19±2.29 |
10 mg/kg Cd | 0 | 31.78±1.64 | 4.82±1.32 | 6.82±1.00 | 56.58±1.69 |
Control | 4 | 31.71±1.73 | 4.68±1.22 | 6.46±1.24 | 57.15±2.22 |
10 mg/kg Cd | 4 | 30.76±1.50 | 4.68±1.17 | 6.93±0.95 | 57.63±1.88 |
Control | 12 | 32.18±2.35 | 4.30±0.96 | 7.81±1.29 | 55.70±1.67 |
10 mg/kg Cd | 12 | 31.55±1.36 | 4.26±0.93 | 7.66±1.22 | 56.53±1.63 |
Replica II | Time point (weeks) | Unmethylated | External C methylation | Internal C methylation | Hypermethylated |
Control | 0 | 31.01±1.68 | 4.51±1.04 | 6.26±0.90 | 58.22±1.81 |
10 mg/kg Cd | 0 | 30.93±1.42 | 4.99±1.26 | 6.42±1.17 | 57.66±1.92 |
Control | 4 | 30.63±2.12 | 4.61±1.11 | 5.53±1.03 | 59.23±1.83 |
10 mg/kg Cd | 4 | 30.30±1.67 | 4.52±0.97 | 5.99±1.06 | 59.19±2.18 |
Control | 12 | 30.44±1.94 | 5.04±1.27 | 7.05±1.10 | 57.47±2.00 |
10 mg/kg Cd | 12 | 30.35±1.63 | 5.20±1.01 | 7.06±1.10 | 57.39±1.63 |
Proportion of unmethylated/methylated fragments across 407 MSL in replica I and 463 MSL in replica II (mean±SD) during the course of the exposure. The number of investigated earthworms in each treatment group of Replica I and II was n = 24.