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. 2015 Jul 22;156(10):3548–3558. doi: 10.1210/en.2015-1120

Figure 5. Estimation of Doc2b-syntaxin 4 and Tctex1d2-syntaxin 4 interaction.

Figure 5.

A and B, 3T3–L1 adipocytes were either untreated or incubated with 100 nM insulin for 30 minutes as indicated. Cell extracts were prepared and immunoblotted (IB) for syntaxin 4 and Tctex1d2. In parallel, syntaxin 4 was immunoprecipitated (IP), and the resultant precipitates were immunoblotted for syntaxin 4 and Tctex1d2. This is a representative immunoblot performed independently 3 times. C and D, 3T3–L1 adipocytes were transfected with either the empty vector (pcDNA3.1) or pcDNA3.1-Doc2b as described under Materials and Methods. Exogenous Doc2b expression was adjusted to a as similar extent with endogenous Doc2b expression. The cells were either untreated or incubated with 100 nM insulin for 5, 15, and 30 minutes as indicated. Cell extracts were prepared and immunoblotted for Doc2b and syntaxin 4. In parallel, syntaxin 4 was immunoprecipitated, and the resultant precipitates were immunoblotted for Doc2b and syntaxin 4. These are representative immunoblots performed independently 3 times. H.C., heavy chain; A.U., arbitrary units.