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. 2017 Apr 20;12(4):e0176246. doi: 10.1371/journal.pone.0176246

Fig 1. Binding site polymorphism from SNP rs2929970 [G/A] in human WISP1 3’-UTR mRNA with microRNA hsa-miR-99a-5p to decrease oral cancer susceptibility among Taiwan HNSCC population.

Fig 1

(A) Exons of WISP1 are shown by the filled boxes from the chromosome positions (chr.13, reference genome GRCh37.p13). (B) The stem-loop portion of miRNA-miRNA duplex structure on pre-miRNAs (hsa-miR-99a; miRBase ID: MI0003190) was identified by microRNA target prediction on MicroRNA.org resource. The hsa-miR-99a-5p sequence marked by green fonts. (C) Sequence of the human WISP1 3’-UTR region and number shown the positions of mRNA (NM_003882). Predicted hsa-miR-99a-5p binding site with SNP rs2929970 was highlighted by color red fonts. (D) The models of microRNA-target duplex were determined using the RNAhybrid web tool on the Bielefeld Bioinformatics Server. RISC, RNA-induced silencing complex, arrows indicate the locus of rs2929970. (E) The SNP rs2929970 A-allele reduces the free binding energy (MFE, minimum free energy; change: 22.16%). (F) Boxplot chart counting the differential expressions of microRNA hsa-miR-99a-5p in the 420 OSCC patients and 43 normal from Pan-Cancer dataset.