No fossil calibration was performed thus the absolute timing of the events cannot be estimated. Most genera were recovered as paraphyletic in [45], except Capoeta, Pseudobarbus, Schizothorax and Sinocyclocheilus, genera for which monophyly had been documented before. Lack of monophyly prevents the estimation of the sampling ratio of each subtrees, thus the analysis were run without sampling information. Panel A: results comparing a diploid clade (black), encompassing Gara and Labeo genera, with a tetraploid one (dark green) encompassing the Barbus, Labeobarbus, Neolissochilus, Varicorhinus and Tor genera. Panel B: subtrees encompassing Pseudobarbus and Barbus species. Panel C: subtrees with hexaploid Capoeta genus members and tetraploid Barbus and Luciobarbus species. Panel D: results from a subtree of panel A, comprising Casobarbus genera and some Barbus species in the tetraploid subclade (black), and hexaploid species of Labeobarbus and Barbus genera (dark green). Panel E: comparison inside the Schizothorax genus.