Table 1. Relative glycolysis and TCA cycle related metabolites in ONS-76 and -F8 cells with and without DCA.
| ONS-76 | ONS-76 +DCA | ONS-F8 | ONS-F8+DCA | Comparative Analysis* | |
|---|---|---|---|---|---|
| Glucose 6-phosphate | 1 | 13.16 | 0.86 | 10.61 | 2,3,4 |
| Fructose 6-phosphate | 1 | 12.21 | 0.70 | 8.54 | 2,3,4 |
| Fructose 1,6-diphosphate | 1 | 13.83 | 1.15 | 14.17 | 1,2,3 |
| Glyceraldehyde 3-phosphate | N.D. | >43.66 | >6.56 | >50.24 | |
| Dihydroxyacetone phosphate | N.D. | >92.31 | N.D. | >101.7 | |
| 2,3-Diphosphoglyceric acid | 1 | 1.69 | 0.79 | 1.59 | 1,2,3 |
| 3-Phosphoglyceric acid | 1 | 0.94 | 1.46 | 0.85 | 1,3 |
| 2-Phosphoglyceric acid | 1 | 1.01 | 1.41 | 1.01 | 1 |
| Phosphoenolpyruvic acid | 1 | N.D. | 5.20 | N.D. | 1 |
| Pyruvic acid | 1 | 2.60 | 0.75 | 1.89 | 2 |
| Intracellular lactic acid | 1 | 14.11 | 1.33 | 13.88 | 2,3 |
| Acetyl CoA | 1 | 22.86 | 0.78 | 21.71 | 2 |
| Citric acid | 1 | 0.99 | 0.87 | 0.80 | 1 |
| cis-Aconitic acid | 1 | 0.94 | 0.96 | 0.76 | 3 |
| Isocitric acid | 1 | 0.60 | 1.34 | 0.63 | 1,3 |
| 2-Oxoglutaric acid | 1 | N.D. | 2.70 | N.D. | |
| Succinic acid | 1 | 0.59 | 0.77 | 0.49 | 1,2,3 |
| Fumaric acid | 1 | 2.77 | 2.29 | 2.37 | |
| Malic acid | 1 | 0.68 | 1.09 | 0.73 | |
| ATP | 1 | 0.76 | 0.77 | 0.60 | 1,2,3,4 |
| ADP | 1 | 1.31 | 0.87 | 1.07 | |
| AMP | 1 | 1.23 | 0.92 | 0.92 | 2,4 |
| Total Adenylate | 1 | 0.78 | 0.77 | 0.62 | 1,2,3,4 |
| ATP/ADP | 1 | 0.59 | 0.91 | 0.56 | 2,3 |
| Adenylate Energy Charge | 1 | 0.99 | 1 | 0.98 | 2,3 |
| NADH | 1 | 1.32 | 0.85 | 1.07 | 1,2,3,4 |
| NAD+ | 1 | 0.97 | 0.80 | 0.71 | 1,3,4 |
| NADH/ NAD+ | 1 | 1.36 | 1.06 | 1.52 | 2,3 |
| Alanin | 1 | 0.71 | 0.85 | 0.43 | 1,2,3,4 |
| Arginine | 1 | 1.29 | 0.83 | 1.08 | 1,2,3,4 |
| Asparagine | 1 | 7.08 | 0.80 | 5.12 | 1,2,3,4 |
| Aspartic Acid | 1 | 2.64 | 0.65 | 1.85 | 1,2,3,4 |
| Cysteine | 1 | 0.30 | 0.75 | 0.34 | 1,2,3 |
| Glutamine | 1 | 64.96 | 1.19 | 58.65 | 2,3 |
| Glutamic Acid | 1 | 1.71 | 1.01 | 1.36 | 2,3,4 |
| Glycine | 1 | 2.37 | 0.28 | 1.46 | 1,2,3,4 |
| Histidine | 1 | 2.35 | 0.90 | 2.21 | 1,2,3,4 |
| Isoleucine | 1 | 3.22 | 0.90 | 2.89 | 1,2,3,4 |
| Leucine | 1 | 3.53 | 0.90 | 3.26 | 1,2,3 |
| Lysine | 1 | 1.68 | 0.83 | 1.40 | 1,2,3,4 |
| Methionine | 1 | 4.96 | 0.94 | 5.06 | 2,3 |
| Phenylalanine | 1 | 2.52 | 0.92 | 2.37 | 2,3 |
| Proline | 1 | 1.95 | 0.75 | 1.57 | 1,2,3,4 |
| Serine | 1 | 1.75 | 0.31 | 0.84 | 1,2,3,4 |
| Threonine | 1 | 1.91 | 0.91 | 1.62 | 1,2,3,4 |
| Tryptophan | 1 | 2.75 | 0.89 | 2.69 | 1,2,3 |
| Tyrosine | 1 | 2.50 | 0.92 | 2.34 | 2,3 |
| Valine | 1 | 2.63 | 0.93 | 2.37 | 1,2,3 |
*: Comparative analysis was performed by Welch’s t test. A P value less than 0.05 was considered statistically significant.
1: significant difference in ONS-76 vs. ONS-F8
2: significant difference in ONS-76 vs. ONS-76+DCA
3: significant difference in ONS-F8 vs. ONS-F8+DCA
4: significant difference in ONS-76+DCA vs. ONS-F8+DCA