Figure 4.

Targeted transcriptional activation of luciferase reporter gene by transient expression of GBD-ROS1. Cells were co-transfected with the indicated effector construct and either the targeted (A) or non-targeted (B) version of the in vitro methylated reporter plasmid. Levels of firefly luciferase transcript, assessed 48 h after transfection by qRT-PCR and normalized to GAPDH, are shown relative to those detected after co-transfection with empty vector and unmethylated reporter. Values are means ± SE (error bars) from three independent transfection experiments. Asterisks indicate statistically significant differences (P < 0.05; Student's unpaired t-test).