Skip to main content
. 2017 Apr 20;12(4):e0174826. doi: 10.1371/journal.pone.0174826

Fig 1. S426A/S430A mutants prefer and consume more ethanol.

Fig 1

A. Consumption (g/kg) of a low (6%) concentration of ethanol was increased for S426A/S430A mutants (red circles and line) relative to wild-type littermates (black lines and squares). Mice were given 24 hour unlimited access to 3%, 6%, 9%, 12%, 15%, and 18% ethanol (v/v) using a two-bottle choice assay. B. S426A/S430A mutants also prefer 6% ethanol more than wild-type littermates. C. Total liquid intake (water plus ethanol) was identical for S426A/S430A mutants and wild-type mice. D. Consumption of 1.75% and 4.25% sucrose was equivalent for S426A/S430A mutants (red bars) and wild-type littermates (black bars). E. S426A/S430A mutants (red bars) and wild-type littermates (black bars) also consume similar amounts of 0.03 mM and 0.1 mM quinine solutions. Averages across the 8 days of intake are shown for each concentration; for both A and B, intakes (collected every 48 hours) are shown in the inset for 6% ethanol. Error bars represent the SEM and data analyses were performed using two-way repeated-measure ANOVA with Bonferroni post-hoc tests (*p<0.05). Sample sizes for each group are in parentheses.