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. Author manuscript; available in PMC: 2017 May 21.
Published in final edited form as: Oncogene. 2016 Nov 21;36(16):2319–2327. doi: 10.1038/onc.2016.397

Figure 2. Knockdown or inhibition of EP4 signaling decreases estrogen independent cell proliferation.

Figure 2

(a) Proliferation of MCF7 cells, which express little EP4, treated with EP4 siRNA and MCF7-LTED cells relative to cells treated with negative control siRNA. (b) RT-qPCR analysis of PTGER4 expression decreases in MCF7-LTED cells treated with two distinct EP4 siRNAs relative to control siRNA. Error bars are standard deviation for three technical replicates. (c) cAMP levels of MCF7-LTED cells treated with EP4 agonist decrease in cells treated with siRNAs to EP4 relative to siRNA controls. (d) Immunofluorescence images of EP4 (green) and DAPI (blue) in MCF7-LTED cells treated with siRNAs targeting EP4 and siRNA controls. (e) Cell proliferation of EP4 antagonists in MCF7 and MCF7-LTED cells relative to cells treated with vehicle only. *** indicates p < 0.001. Error bars show s.e.m. of three replicates.