Figure 6.

Wwox significantly decreases Rpa32 and Rad51 foci formation after IR. Representative immunofluorescence images of γH2AX, RIF1, 53BP1, Brca1, Rpa32 and Rad51 foci in 231/Wwox cells untreated or treated with doxycycline (24 h) at various time points after 10 Gy IR (left). Wwox expression significantly decreases Rpa32 and Rad51 foci 1 h after IR and increases γH2AX foci 3 h after IR. Graphs (right) represent protein foci averages of three independent experiments. Foci were quantified using the Image J software (Bethesda, MD, USA). Experiments were performed in triplicate and statistical significance was determined using the two-tailed Student's t-test and error bars depict standard error.