Fig. 1.

Schematic of the methodology used in this study. Wild-caught males were utilized for the liver microsomal EROD assay and were also bred with wild-caught females to obtain the F1 generation. F1 embryos were either exposed at 24 hpf to PAH mixtures and utilized during the embryo EROD, deformity scoring, and/or bioenergetics assessments or raised under normal conditions for 6 months. These 6 month old juvenile fish were then utilized for whole-organism metabolic testing and then sacrificed for tissue bioenergetics testing