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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Addict Biol. 2016 Oct 20;22(6):1856–1869. doi: 10.1111/adb.12464

Figure 9. Moderate consumption of alcohol as well as sucrose consumption do not activate AKT/mTORC1 signaling in the orbitofrontal cortex.

Figure 9

(a) Phosphorylation of AKT and S6 in the OFC of rats after 3 weeks of CA10%-2BC was determined by western blot analysis. Phosphorylation of AKT and S6 was determined by western blot analysis. ImageJ was used for optical density quantification. Data are expressed as the average ratio +/− S.E.M of phospho-AKT to AKT and phospho-S6 to S6 and are expressed as percentage of water (W) controls. Significance was determined using two-tailed unpaired t-test. pAKT p>0.05; pS6 p>0.05. n=4 per group. (b) Phosphorylation of AKT and S6 in the OFC after 7–8 weeks of IA1%-2BC of sucrose was determined by western blot analysis. pAKT p>0.05; pS6 p>0.05. n=5 for sucrose group, n=4 for water group.