Fig. 2.

Minocycline (Mino/Minocyc) partially reduced the activation of calcineurin (CaN) in prion-infected hamsters. (a) Representative blots of 2 animals per group PK^– or PK⁺. Eight animals from each group were used for Western blot analysis. (b) CaN activity in response to the accumulation of misfolded proteins: day 85 and day 125 results of all groups; number of animals tested per group was 3 for each experiment. Data were analyzed by 2-way analysis of variance (ANOVA) with Bonferroni’s post-test (***p < 0.001). (C) Representative blots of 2 animals per group for the regulatory unit of CaN expression. (d) Statistical analysis of CaN B expression in brain homogenates from different groups; number of animals used for statistical analysis per group was 5; and the data were analyzed by 1-way ANOVA with Tukey’s multiple comparison post-test (***p < 0.0001). (e) Representative blots from 2 animals per group for the catalytic unit of CaN expression. (f) Statistical analysis of CaN A expression in brain homogenates from different groups; number of animals used for statistical analysis per group was 5; and the data were analyzed by 1-way ANOVA with Tukey’s multiple comparison post-test (***p < 0.0001)