Fig. 7.

Minocycline (Mino) effectively leads to reduced nuclear factor kappa B (NF-κB) p65 nuclear translocation. (a) Representative Western blot of 2 animals from every group for the expression of NF-κB p65 in cytoplasmic extracts (upper panel) in comparison with β-actin levels and nuclear extracts (lower panel) in comparison with nucleophosmin levels. (b) The data showing NF-κB p65 expression levels in cytoplasm and nucleus of different experimental groups are based on 5 animals per group. Data were analyzed by 2-way analysis of variance with Bonferroni’s post-test (***p < 0.001). (c) Representative pictures of confocal microscopy for nuclear translocation of NF-κB in different groups, First panel stained for nucleus with 4,6’-diamidino-phenylindole (DAPI; blue), second panel stained for NF-κB p65 (red), third panel is a merger of first and second panel, and the last panel shows the high magnification of the merged image. NPM = Nucleophosmin