Fig. 1.

ACY-738 and ACY-775 are potent and selective histone deacetylase 6 inhibitors. (a) N2a cells were incubated with 1 μM ACY-738, ACY-775, or trichostatin A (TSA) as a positive control. By Western blot the acetylation of α-tubulin (acet α-tub) was assessed, as well as the acetylation of histone 3 (acetH3). Total α-tubulin levels, glyceraldehyde 3-phosphate dehydrogenase (gapdh) and histone 4 (H4) were used as a reference for equal loading. (b) The ratio of acetylated α-tubulin to total tubulin levels was calculated and normalized to TSA-treated cells. One-way analysis of variance (ANOVA); ***p < 0.0001; n = 4. (c) The ratio of acetylated histone 3 to histone 4 levels was assessed and subsequently normalized to vehicle-treated cells. One-way ANOVA; **p < 0.01; n = 3–4. (d–g) Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775