Figure 3.

Clustal alignment of the sequences of the CsFAD2R1 target site in Camelina sativa leaf sample T1‐1‐3 exhibiting Cas9/sgRNA‐mediated gene editing. Illumina Amplicon‐Seq was used for DNA sequencing. Read frequency counts for each sample are provided to the left of the sequences. Only the most common sequences found at least 50 times are depicted. Asterisks denote nucleotides conserved in all three subgenome homoeologs (A, B and C). Lack of an asterisk denotes a nucleotide position at which there is a single‐nucleotide polymorphism in one of the three homoeologs.