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Seed oil profile of seeds from Arabidopsis plants transformed using Cas9/sgRNA targeting the R2 site of the AtFAD2 gene. Blue: wild‐type control; green: T3 seeds (from T2 plant T1‐25‐4); light red: T4 seeds (from T3 plant, in order, T1‐25‐4‐1, T1‐25‐4‐2, T1‐25‐4‐3, T1‐25‐4‐4, T1‐25‐4‐5) derived from T1‐25‐4. Methods for extraction and measurement of Arabidopsis seed fatty acids are detailed in Experimental procedures.
