Table 3.
Ammonia production by the kivd + ΔglnA strain with various nitrogen sources (7.25 g/L)
| Nitrogen source | Cell density OD600 | Produced ammonia by E. coli (mg/L) | Theoretical amount of maximum ammonia production (mg/L)a | Yield (%) |
|---|---|---|---|---|
| Yeast extract (M9_YE) | 1.51 ± 0.05 | 439 ± 12 | 960 | 45.8 |
| Tryptone | 1.25 ± 0.08 | 427 ± 25 | 1171 | 36.5 |
| Peptone | 1.00 ± 0.06 | 375 ± 10 | 1356 | 27.7 |
| Casamino acids | 0.99 ± 0.03 | 453 ± 35 | 951 | 47.6 |
Values of yield were calculated as produced ammonia by E. coli per theoretical amount of maximum ammonia production. Values given as mean ± SD (n = 3)
aThe values were calculated based on the nitrogen contents of BD Bionutrients™ Technical Manual Third edition (http://www.bd.com/ds/technicalCenter/misc/lcn01558-bionutrients-manual.pdf.)