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. 2017 Apr 21;8:461. doi: 10.3389/fimmu.2017.00461

Figure 1.

Figure 1

Human cytomegalovirus (HCMV)-infected mature DCs (mDCs) show decreased transwell migration toward CCL19. (A) Mature dendritic cells were mock-, HCMV- or HCMV-ultraviolet (UV)-infected and analyzed for infection rates at the indicated time points. Mock or HCMV/green fluorescence protein (GFP)-negative mDCs are indicated as black dots, while HCMV/GFP-positive cells are marked in green. (B). Mock- (white columns), HCMV-infected mDCs (HCMV-positive cells are depicted in green and HCMV-negative cells in black columns), and HCMV-UV-infected mDCs (gray columns) were subjected to a transwell migration assay on fibronectin-coated transwell inserts 24 hpi. To induce chemotaxis the chemokines CCL19 or CXCL12 (100 ng/ml) were added to the lower wells. As control no chemokine was added for spontaneous migration, indicated as “w/o.” Migration was evaluated after 2 h, and the percentage of migrated mDCs was calculated in the lower well by determining cell number and infection rates of migrated cells in relation to the respective starting condition. The experiment was performed at least three times with cells from different healthy donors. Significant changes (** = p < 0.01) are indicated by asterisks.