Figure 4.

Human cytomegalovirus (HCMV) infection of mature dendritic cells (mDCs) leads to decreased CYTIP expression levels. (A) mDCs were either mock- or herpes simplex virus type 1 (HSV-1)-infected and harvested 24 hpi, (B) mock- or HCMV-infected and sorted based on their green fluorescence protein expression 24 hpi. (A,B) Protein lysates were prepared and subjected to Western blot analyses with antibodies specific for CYTIP, cytohesin-1, ICP0, IE2, or GAPDH. One representative experiment out of at least three is shown. (C) Quantification of CYTIP and cytohesin-1 protein expression levels in HSV-1-infected or HCMV-positive mDCs, summarizing at least three independent experiments. Expression of CYTIP and cytohesin-1 was normalized to the GAPDH control. Mock was set to 100%. Significant changes (**** = p < 0.0001; *** = p < 0.001) are marked by asterisks, non-significant changes (p > 0.05) as “ns.”