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. 2017 Apr 21;8:461. doi: 10.3389/fimmu.2017.00461

Figure 5.

Figure 5

Human cytomegalovirus (HCMV) induces CYTIP downmodulation early after infection. Mature dendritic cells (mDCs) were mock- or HCMV-infected and harvested at the indicated time points post-infection. Cells were intracellularly stained with antibodies specific for CYTIP or cytohesin-1 and subjected to flow cytometric analyses. (A,C) Representative data of one donor are shown for CYTIP (left panel) or cytohesin-1 (right panel) expression levels 24 hpi. Mock conditions are depicted as black lines (exemplary for 24 hpi), (A) HCMV-positive cells as filled green histograms and (C) HCMV-negative cells as filled black histograms. (B,D) Quantification of CYTIP and cytohesin-1 protein levels 12 hpi (n = 3), 24 hpi (n = 6) and 36 hpi (n = 3). Median values of (B) HCMV-positive (green bars) and (D) HCMV-negative (black bars) mDCs were calculated relative to the respective mock condition (white bars). Significant changes (*** = p < 0.001) are marked by asterisks, non-significant changes (p > 0.05) as “ns.”