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. 2017 Apr 21;8:601. doi: 10.3389/fpls.2017.00601

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Copyright © 2017 Brikis, Zarei, Trobacher, DeEll, Akama, Mullen, Bozzo and Shelp.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of MdGLYRs in tobacco BY-2 cells. Representative CLSM images of BY-2 cells (co)expressing (as indicated by the panel labels) MdGLYR1-GFP (A) and the cytosolic marker Cherry (B) or MdGLYR2-GFP (E) and plastid marker PDC_pl-E2-mCherry (F). Processing of cells and viewing using CLSM are described in the Section ‘Materials and Methods’. Shown also is the corresponding merged (C,G) and differential inference contrast (DIC) (D,H) images for each cell. The yellow color in the merged images indicates co-localization. Scale bar in A = 10 μm.