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. 2017 Apr 21;8:231. doi: 10.3389/fphys.2017.00231

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Copyright © 2017 Soon, Abdul Murad, Then, Abu Bakar, Fadzil, Thanabalan, Mohd Haspani, Toh, Mohd Tamil, Harun, Wan Ngah and Jamal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative mitochondrial functions in different grades of glioma cell lines and normal human astrocyte. (A) Bar chart showing significantly lower level of mitochondrial function:mass ratio in grade II 1321N1 and grade III SW1783 cell line (^*indicates p < 0.05 using one-way ANOVA, bars represent mean MTO:MTG pixel intensity index ± 1 SEM, mean index value as shown in bars). (B) Relative mitochondrial functions were demonstrated using MitoTracker Orange (MTO) stain where relative mitochondrial mass was shown by MitoTracker Green (MTG) stain. Each stain was captured using CLSM with sequential detection. Images merged and analyzed quantitatively using LAS AF software to generate the relative function ratio.