Figure 4.

Oxidative stress status in different grades of glioma cell lines. (A) A significantly higher total antioxidant capacity demonstrated by LN18 glioblastoma multiforme cells (p < 0.05 using one-way ANOVA). (B) The DNA oxidation marker, 8-OHdG level was higher in grade II 1321N1 cells which was also harboring the most coding region non-synonymous mtDNA mutations (^*indicates p < 0.05 using one-way ANOVA, bars represent mean 8-OHdG concentration ± 1 SEM, mean values as shown in bars). (C) Direct staining of CM-H₂DCFDA in live cells showed that grade II 1321N1 cells sustained highest level of oxidative stress in-vitro (^*indicates p < 0.05 using one-way ANOVA, bars represent mean pixel intensity ± 1 SEM, mean values as shown in bars). (D) Images of cells stained with CM-H₂DCFDA and DAPI clearly demonstrated that 1321N1 cells harbored the highest amount of oxidative stress marker. As compared to the other cell lines, some cells detected by DAPI did not showed any green fluorescence.