Figure 3.

Frameshift mutation in pe_pgrs33 negatively affects the protein-mediated entry of Mtb into murine macrophages. pMMOs were infected at a MOI of 1:10 with Mtb H37Rv, the MtbΔ33 mutant and a panel of complemented strains (MtbΔ33::33^all11, MtbΔ33::33^all3, MtbΔ33::33^all5, MtbΔ33::33^all6, and MtbΔ33::33^all18). For all Mtb strains, mean value of CFUs in triplicate from a representative experiment of pMMOs infection and the respective standard deviation are represented. ^**p < 0.01 and ^***p < 0.001 compared to Mtb H37Rv (one-way ANOVA followed by Dunnett's multiple comparison test).