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. 2017 Apr 21;7:137. doi: 10.3389/fcimb.2017.00137

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Copyright © 2017 Camassa, Palucci, Iantomasi, Cubeddu, Minerva, De Maio, Jouny, Petruccioli, Goletti, Ria, Sali, Sanguinetti, Manganelli, Rocca, Brodin and Delogu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mtb lacking PE_PGRS33 or expressing the truncated allele 3 mainly localizes extracellularly in murine lungs during the chronic/persistent TB stages. Cellular localization of bacilli was investigated at day 49 post-infection on lung sections of mice infected with the Mtb H37Rv (A,B), MtbΔ33::33^all3 (C,D) and MtbΔ33 (E,F) strains, by performing immunofluorescence assay (B,D,F) and comparing results with those obtained from observation of ZN-stained lung sections (A,C,E). Immunofluorescence was carried out by using anti-F4/80 and anti-MT 16 kDa antigen, as markers of macrophages (green) and bacilli (red), respectively. Colocalization of macrophages and bacteria resulted in yellow signals. Representative images are shown.