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. 2017 Apr 21;8:692. doi: 10.3389/fmicb.2017.00692

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Copyright © 2017 Cheng, Yang, Wang, Lin and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cellular localization by immunofluorescence confocal microscopy. (A) Plasmids expressing EGFP-tagged PB1-F2 of HK156 and PR8, as well as HK156-ILVF and PR8-TQDS, two swapping mutants, were transfected into HeLa cells. Cells were stained with DAPI (blue) for nuclei and MitoTracker Orange (red) for mitochondria. EGFP signal indicates location of PB1-F2 protein (green). Yellow color indicates merge of red and green signals. Images were captured using Objective Plan-Apochromat 1.4 Oil DIC M27 with power of 63× (Left) or 100× (Right) with Zeiss LSM700. (B) Mitochondrial localization of denoted PB1-F2 was calculated as the percentage of signals of EGFP/MitoTracker. n, number of cells observed from 10 randomly selected fields.