FIGURE 5.

IFN-β modulation by PB1-F2 variants. Plasmids expressing PB1-F2 of PR8, HK156, PR8-TQDS, and HK156-ILVF were individually cotransfected with p125-luc, a reporter for IFN-β promoter activity, into HEK293T cells. 24 h post-transfection, cells were infected with Sendai virus (SeV) to induce IFN-β response. Infected cells were harvested 24 h post-infection for p125-luc reporter assay. Mean values of luciferase activity of two independent experiments with three readings each were shown. (^∗p < 0.05; ^∗∗p < 0.01, Student’s t-test).