Table 1.
Primer used for constructing PB1-F2 expressing plasmids.
| Flanking primers |
Internal primers |
||||
|---|---|---|---|---|---|
| Forward | Reverse | Forward | Reverse | Template | |
| pFLAG-HP1 | No. 621 | No. 622 | No. 613 | No. 614 | pFLAG-PB1-F2 (PR8) pFLAG-PB1-F2 (HK156) |
| pFLAG-HP2 | No. 621 | No. 622 | No. 615 No. 617 | No. 616 No. 618 | |
| pFLAG-HP3 | No. 621 | No. 622 | No. 619 | No. 620 | |
| pFLAG-HK156-ILVF | No. 797 | No. 798 | – | – | pLNFG-HK156-ILVF-F2 |
| pFLAG-PR8-TQDS | No. 795 | No. 796 | – | – | pLNFG-PR8-TQDS-F2 |
| pEGFP-PB1-F2 (HK156) | No. 675 | No. 622 | – | – | pFLAG-PB1-F2 (PR8) |
| pEGFP-PB1-F2 (PR8) | No. 674 | No. 622 | – | – | pFLAG-PB1-F2 (HK156) |
| pEGFP-HK156-ILVF | No. 675 | No. 622 | No. 738 | No. 737 | pFLAG-PB1-F2 (PR8) pFLAG-PB1-F2 (HK156) |
| pEGFP-PR8-TQDS | No. 674 | No. 622 | No. 746 | No. 747 | |
| pHW192-PB1-F2-TQES | No. 683 | No. 684 | No. 766 | No. 767 | pHW192 |
Vector for EGFP-tagged PB1-F2: pLNFG; Vector for FLAG-tagged PB1-F2: pFLAG-CMV2.