Figure 1.

Structural model of scR1antTNFs. A, a structural model of scR1antTNF-L1 (GGGGS linker) with a TNFR1 extracellular domain was constructed using Modeller. The R1antTNF was composed of three monomers (gray schematic). The N- and C-terminal peptide linkers are shown as a green schematic. Monomeric TNFR1, which interacts with the two TNF monomers, is shown as a red schematic. For clarity, only two parts of the three monomers are shown. Amino acids mutated in R1antTNF that are thought to interact with TNFR1 are shown as orange spheres. The peptide linkers of scR1antTNF-L2 (GGGSGGG) and scR1antTNF-L3 (GGGSGGGSGGG) are shown as red and blue schematics, respectively. Verify 3D (B) and ERRAT (C) were used to check the quality of the protein models. The structural models of scR1antTNF with three different linker sequences, GGGGS (gray line), GGGSGGG (dotted line), and GGGSGGGSGGG (black line), were compared using Verify 3D.