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EPR spectroscopic characterization of H64A FeIII-Ngb following the addition of Na2S. Shown is the spectrum of H64A FeIII-Ngb (500 μm) in aerobic 100 mm HEPES, pH 7.4, and 20% glycerol (v/v) after the addition of Na2S (10 mm) at 25 °C at the indicated times. The EPR settings are described under “Experimental procedures.” The signal marked with an asterisk represents adventitious iron. R.U., relative units.
