Figure 6.

NOSTRIN inhibits VEGF-induced tube formation and adhesion of macrophages to endothelial cells. A, photomicrograph of tube formation in the four treatment groups at 12 h following plating of cells on basement membrane matrix-coated plates. Experiments were repeated three times with different biological samples. B, quantification of capillary tube formation in four treatment groups using data from three replicate experiments and five random microscopic fields per experiment. Data were analyzed using Wimasis image analysis software. Error bars represent S.E. C, photomicrograph images of calcein-labeled macrophages adhered to endothelial cell monolayer from four treatment groups. D, quantification of fluorescence of the adhered macrophages normalized to total number of endothelial cells available per well. *, p < 0.05; and **, p < 0.01.