Figure 10.

The combination of IL-6 and EBI3 induces chemokine expression and production in HUVEC in a dose-dependent manner, and this expression is blocked by anti-gp130 mAb. A, cells were stimulated for 4 h with EBI3 (1 μg/ml) and sIL-6R (100 ng/ml) with or without IL-6 (10 ng/ml). Gene expression was assessed by real-time RT-PCR. B, cells were stimulated for 4 h with IL-6 (10 ng/ml) and an increasing concentrations of EBI3. Gene expression was assessed by real-time RT-PCR. C and D, cells were stimulated for 24 h with EBI3 (2 μg/ml) and IL-6 (10 ng/ml) or with EBI3-IL-6 recombinant fusion protein (200 ng/ml) with or without anti-gp130 mAb. Chemokine levels were measured by ELISA. Statistical differences between sIL-6R ± IL-6 and EBI3 ± IL-6 were determined using a Student's t test. ***, p < 0.001; *, p < 0.05. The error bars in the figures represent S.D. of triplicate cultures. The data are representative of two independent experiments.