Figure 3.

EBI3-induced B9 cell proliferation can be inhibited by anti-IL-6, anti-gp130, and 25F10, a mAb that block IL-6 trans-signaling. Untransfected B9 cells were incubated with mEBI3 (500 ng/ml), IL-6 (0.01 ng/ml), or IL-27 (100 ng/ml) with or without anti-gp130 (A), anti-IL-6 (B), 25F10 (C), or anti-IL-6R (D) mAbs. Proliferation was measured as described in the legend to Fig. 2. Error bars indicate the S.D. of triplicate cultures. Statistical differences between IL-6 ± mAbs and EBI3 ± mAbs were determined using Student's t test. ***, p < 0.001; **, p < 0.01. The data are representative of three independent experiments.