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. 2017 Mar 9;292(16):6644–6656. doi: 10.1074/jbc.M116.762021

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 9. — EBI3-IL-6 fusion interaction with soluble gp130 assessed by SPR. Soluble gp130 was immobilized via EDC/NHS on 50-mm-thick gold surface coated with a peptide monolayer as indicated under “Experimental procedures.” The sensorgram for EBI3-IL-6 (A and C) or EBI3 (B and C) are indicated. PBS (pH 7.4 or 4.5) rinsing is also shown. The decrease of the SPR signal observed with the injection of EBI3 is due to the different refractive index of the solution. The data are representative of three independent experiments.